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AIM:To elucidate the molecular mechanisms of lipoic acid (LA) on redox regulation and digestive function in intestine of C57BL/6 mice fed high fat diet (HFD).METHODS:C57BL/6 mice were randomly assigned to one of three groups (n=8).The control group consumed an ordinary diet.The other two experimental groups were fed with a high fat diet, high fat plus 0.1% LA.After 6 weeks, the activities of digestive enzymes were examined.In order to evaluate the antioxidant status of the mice, superoxide dismutase (SOD), malondialdehyde (MDA), total antioxidant capacity (TAC) and reactive oxygen species (ROS) in intestinal homogenate were measured.To investigate the molecular mechanisms underlying the effects of LA, the gene expression profiles in intestine were examined using the GeneChip microarray system.RESULTS:A depressed antioxidant defense system, accompanied by digestive and absorptive function impairment, was observed in HFD-fed mice.These changes were partially restored in the LA-treated group.DNA microarray analysis of intestine showed that LA ingestion up-regulated the expression of genes related to free-radical scavenger enzymes, digestive enzymes and transporters.CONCLUSION:Treatment with LA improves redox homeostasis and the function of intestine in mice fed HFD.The mechanism may involve preventing oxidative stress by scavenging ROS directly and increasing those of free-radical scavenger enzymes gene expression indirectly. 相似文献
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关于曲线在一点的基本三棱形的探讨 总被引:1,自引:0,他引:1
以弧长为参数,定义了曲线在一点的弗雷内标架与基本三棱形,推导出其对应量的一般参数方程. 相似文献
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给出了用组合数学中的递推关系H_n=■α_iH_(n-i)(其中α_1,α_2,…,α_r为常数)计算具有二阶及二阶以上常系数递推关系的n阶行列式的计算方法.通过实例给出此方法的解题步骤,并验证了其可行性. 相似文献
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饱和度指标可通过含水率换算获得;但对长期浸水的大堤而言,由于采用烘干法试验测得的试样的含水率普遍偏高,因此直接计算的饱和度值普遍偏大;根据土、水相互作用性质,分析土颗粒周围结合水分布特点,指出浸水大堤填土试样饱和度偏高的原因在于烘干试验中有部分弱结合水逸出,并且这部分弱结合水被计入到重力水中;根据经典土力学理论,建立完全饱和土的二相体系模型,对饱和度与弱结合水含量间的关系进行定量分析,并把所得的结论用于工程实践。 相似文献
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ZHANG Shengying WU Xiaochun XING Xiaoyong LIU Jia YUE Yahui ZHANG Yangyang HE Jian WEN Fengqin BAO Shijun 《中国畜牧兽医》2007,47(10):3149-3157
The aim of this study was to investigate the effect of P48 protein on proliferation and apoptosis of embryonic bovine lung (EBL) cells.In this study,samples which co-incubated with P48 protein and EBL cells in different concentrations at different time points were collected,the proliferation rate of the cells was detected by MTT method,and the changes in the nuclear morphology of EBL cells were observed by DAPI staining method.Meanwhile,flow cytometry was used to detect the apoptosis rate of EBL cells induced by P48 protein.Real-time quantitative PCR was used to detect the changes in mRNA level of apoptotic marker genes,and Western blotting tested the Bax and Beclin-1 protein expressions.The results showed that under the condition of 72 h and 10 μg/mL of protein concentration treatment,P48 extremely significantly inhibited EBL cells proliferation (P<0.01),while 0.1 and 0.5 μg/mL protein concentration had no inhibitory effect (P>0.05).The nuclear morphology showed no significant change after protein induction for 12 h,but wrinkled and condensed at 24 h.The nucleus was fragmented,and a sprouted apoptotic body was appeared at 48 and 72 h.Apoptosis related genes expression showed no obvious increase at 2 and 12 h at mRNA level,but gradually increased at 24,48 and 72 h,and it showed a time-dependent manner.Accordingly,the expression of apoptosis marker proteins Bax and Beclin-1 significantly increased.Flow cytometry analysis showed that the apoptosis rate of EBL cells induced by P48 protein was 48.44%.In conclusion,P48 recombinant protein of Mycoplasmas bovis inhibited the proliferation of EBL cells and promoted their apoptosis,which provided reference for revealing the pathogenic mechanism of Mycoplasmas bovis. 相似文献
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